Background
The growth and reproduction of microorganisms require appropriate temperature and humidity. In particular, fungi prefer a humid and warm environment, and the optimum temperature for fungal growth is around 30 °C. Fungi are present everywhere in the environment such as atmosphere, plants, animals, feces, floors, and soil [
1]. During spring and summer in Southern China, high-temperature and humidity environments are especially suitable for the growth and reproduction of fungi, and thereby increase risk of pathogenic fungal infections in population with long-term exposure to warm and humid environments. Older individuals and patients with chronic respiratory diseases are susceptible to respiratory fungal colonization and invasion [
2]. Generally, fungal infections in the respiratory tract are dominated by
Candida spp., especially
C. albicans [
3].
Detection methods such as 1,3-beta-D-glucan assay (G test) [
4], microscopic examination [
5], culture methods [
6], histopathology [
7], serology [
8], and molecular biology [
9] have been used in diagnosis of fungal infections. Traditional culture methods are widely used to characterize pathogenic fungi including
Candida spp. However, culture-based methods are cumbersome, time-consuming, and thus unsuitable for rapid detection. Even more, the results are generally false negative. G test is used to detect one of the main structural components of the fungal cell wall (1,3-beta-D-glucan), but cannot distinguish fungal species. Despite the accuracy and efficacy of PCR-based methods [
10], using a pair of primers or probes can only detect one type of the fungi; even multiplex PCR can detect no more than 5
Candida spp [
11]. Therefore, bio-chips including microfluidic chip are becoming an alternative and revolutionary technology for assaying multiple fungi.
In this study, we aimed to rapidly detect the pathogenic fungi in coastal population with respiratory infectious diseases using microfluidic technology. To verify the accuracy of microfluidic chip, the results were further validated using Real-time qPCR and the clinical baseline data of the patients with or without fungal infections were analyzed.
Methods
Respiratory samples of patients
Patients with severe respiratory infections and hospitalized in the Department of Critical Care Medicine and Department of Respiratory and Critical Care Medicine between July 2020 and June 2021 at the Affiliated Hospital of Guangdong Medical University (Zhanjiang, China) were selected in this study. The patients had to be aged ≥ 18 years and their respiratory samples (sputum, alveolar lavage fluid, pleural fluid, etc.) were collected and sent to the department of precision laboratory for inspection. Cases who refused to accept the chip-based detection of pathogenic fungi were excluded. This study was approved by the ethics committee of the Affiliated Hospital of Guangdong Medical University (approval No. KT2022-119-01) and the consent of all patients was obtained.
Clinical data collection
Clinical baseline data (including age, gender, fever, cough, sputum production, coma, use of antifungal agents, white blood cells, neutrophils, lymphocytes, red blood cells, platelets, glucose, total protein, albumin, and globulin) and microbiological culture results were obtained from electronic medical records (Table
S1).
Microfluidic-based detection of pathogenic fungi
The genomic DNAs of respiratory samples were isolated using the nucleic acid extraction kit (CapitalBio Technology, China) and subsequently used for chip-based detection of pathogenic fungi. The butterfly-shaped microfluidic chip system for rapid detection of broad-spectrum fungi was customized from CapitalBio Technology and prepared by constant temperature amplification with microfluidic technologies. The reaction of microfluidic-based detection was carried out on a microfluidic disk chip. Each chip had 24 reaction pools, in which were embedded with a set of specific primers targeting 20 species of pathogenic fungi. The fluorescent dye incorporation method was used, and the isothermal amplification technology adopted was loop-mediated isothermal amplification (LAMP). Its principle is based on the fact that DNA is in a dynamic equilibrium state at about 65℃. When any primer carries out base pairing extension to the complementary position of double-stranded DNA, the other strand will dissociate and become a single strand. After the one-step amplification and detection of target sequences, the samples generating snake-shaped amplification curves represented positive signals.
Validation using real-time qPCR
According to the detection results of microfluidic chip, the specific primers of pathogenic
Candida spp. including
C. albicans,
C. tropicalis,
C. glabrata,
C. parapsilosis, and
C. krusei were synthesized for qPCR validation. Real-time qPCR was performed according to the specification of 2×RealStar Green Power Mixture with ROX (GenStar, China) by Applied Biosystems 7300 Real-Time PCR System (Thermo, USA). The cycling conditions were as follows: a pre-run at 95 °C for 10 min, 40 cycles of denaturation at 95 °C for 15 s, followed by a 60 °C annealing for 45 s and a 72 °C extension for 30 s; the melting curve conditions were automatically set up. The Real-time qPCR reactions were performed in triplicate and GAPDH was used as internal reference. Finally, the amplified products were electrophoresed on 1.0% agarose gels and developed using the nucleic acid dye Gelred (Biotium, USA). The sequences of PCR primers were listed in Table
S2.
Statistical analysis
The measurement data that conformed to a normal distribution were expressed as mean ± standard deviation (SD), and the t-test was used for the comparison between the two groups; the measurement data that did not conform to the normal distribution were expressed as median and interquartile range (IQR), and the comparison between the two groups was conducted by Mann-Whitney U test. The count data were expressed as the number of cases and percentage, and the difference was analyzed by chi-square test or Fisher’s exact value test. P < 0.05 was considered to demonstrate statistically significant differences.
Discussion
While microfluidic chip has been used for rapid detection of fungal infections [
12], it is still a bottleneck problem to test special clinical samples via high-throughput assays. To detect low-density fungi in body fluid, we customized the microfluidic-based chip system which allows timely detection of broad-spectrum pathogenic fungi, including
C. albicans,
C. tropicalis,
C. glabrata,
C. parapsilosis,
C. krusei,
C. auris,
A. fumigatus,
A. flavus,
A. niger,
C. neoformans,
T. asahii, etc. It is shown that the pathogenic fungi detected by culture method and chip technology mainly consisted of
Candidas, but the detection rate of microfluidic chip technology were higher than that of culture method (Fig.
1). The molecular detection results of
Candidas using the microfluidic chip were validated by Real-time qPCR and showed good agreement (Fig.
2). Moreover, the blood routine examination implies that the platelets counts may be reduced in the patients with
Candida infections and even associated with infection severity (Tables
2 and
3).
Invasive fungal infections are the most common causes of high mortality in ICU, and prompt antifungal therapy is a critical determinant for good clinical outcomes [
13]. However, the treatment of fungal infections is often delayed due to the insensitivity and slow steps of microbial culture. Therefore, the development and validation of non-culture diagnostics targeting pathogenic fungi are of utmost priority in medicine. While commercial PCR detection has been widely applied to fungal examination, the use of DNA chip is becoming the method of choice for profiling fungal gene expression [
14,
15]. The results of microbial culture showed only fungal infections in 16 of 64 patients, in which one was negative by microfluidic chip and qPCR validation (Fig.
1 and
2; Table
1). In contrast, 36 cases that test
Candida positive by microfluidic chip were consistent with the qPCR validation (Figs.
1 and
2; Table
1), suggesting that the sensitivity and accuracy of microfluidic chip technology for fungal detection are more superior than culture methods. Furthermore, the microfluidic chip can simultaneously detect 20 species of pathogenic fungi, suggesting that it has the advantage of high-throughput detection compared with conventional PCR methods.
Candida spp. especially
C. albicans play essential roles in invasive fungal infections. The development of diagnosis and treatment methods increases the risk of
Candida infections [
16‐
18], for instance, the use of broad-spectrum antibiotics, immunosuppressants, total parenteral nutrition, and mechanical ventilation leads to microbial dysbiosis. According to common sense, pathogenic microorganism infections cause blood-routine changes. However, we found that white blood cell counts were not significantly different between the patients infected with
Candida spp. and fungal-negative controls (Table
2), probably due to additional bacterial infections.
As multiple players in innate immunity, platelets interact with
Candida spp. and may contribute to the fatal outcome of invasive
Candida infections [
19,
20]. Thrombocytopenia, an important risk factor for fungal infections was also observed in the patients infected with
Candidas (Table
3). It is suggested that
Candidas enhance platelet adherence [
19]. Platelets cannot directly reflect
Candida infections, but may favor the survival of invasive
Candidas in blood [
21]. We found that platelet counts decreased in some patients with
Candida infections in respiratory tract (Table
3), which is consistent with previous literature reports [
22,
23], suggesting that
Candida spp. may also be present in their blood and hence cause platelet aggregation and destruction. The mechanism by which platelets adhere to
Candidas depends on the species of
Candidas [
20]. A study showed that
C. albicans was able to evade complement-mediated killing without platelet adherence, and thus survived in blood during the infection period [
24]. Moreover, it is showed that thrombocytopenia in the patients co-infected with
C. albicans and other
Candidas was more severe than those infected with
C. albicans alone (Table
3). These findings suggest that platelet counts may be used as a parameter to investigate the severity of invasive
Candida infections. However, the number of the patients in each group is small. Therefore, future studies should expand the sample size to observe and explore the relationship between platelets and
Candida infections.
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