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01.04.2024 | Original Article

Dietary restriction plus exercise change gene expression of Cxcl12 abundant reticular cells in female mice

verfasst von: Aoi Ikedo, Yuuki Imai

Erschienen in: Journal of Bone and Mineral Metabolism

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Abstract

Introduction

Low energy availability due to excessive exercise lowers bone mass and impairs various physiological functions, including immunity and hematopoiesis. We focused on Cxcl12 abundant reticular (CAR) cells, which are bone marrow mesenchymal stem cells and are essential for the maintenance of hematopoietic and immune cells in bone marrow. We examine the functional changes in CAR cells resulting from dietary restriction combined with exercise.

Materials and methods

Five-week-old wild-type female mice were divided into an ad libitum group (CON), a 60% dietary restriction group (DR), an ad libitum with exercise group (CON + ex), and a 60% dietary restriction with exercise group (DR + ex). Blood parameters, bone structure parameters, and bone marrow fat volume were evaluated after 5 weeks. In addition, bone marrow CAR cells were isolated by cell sorting and analyzed for gene expression by RT-qPCR.

Results

Bone mineral density (BMD) was significantly decreased in DR and DR + ex compared to CON and CON + ex. Especially, cortical bone mass and thickness were significantly decreased in DR and DR + ex groups, whereas trabecular bone mass was significantly increased. Bone marrow fat volume was significantly increased in DR and DR + ex groups compared to CON and CON + ex. The number of leukocytes in the blood was significantly decreased in the DR + ex group compared to the other three groups. RT-qPCR showed a significant decrease in gene expression of both Foxc1 and Runx2 in CAR cells of the DR + ex group compared to CON.

Conclusion

Dietary restriction combined with exercise promotes CAR cell differentiation into bone marrow adipocyte and suppresses osteoblast differentiation.

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Titel: Dietary restriction plus exercise change gene expression of Cxcl12 abundant reticular cells in female mice
verfasst von: Aoi Ikedo
Yuuki Imai
Publikationsdatum: 01.04.2024
Verlag: Springer Nature Singapore
Erschienen in: Journal of Bone and Mineral Metabolism
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-024-01506-6

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Dietary restriction plus exercise change gene expression of Cxcl12 abundant reticular cells in female mice

Abstract

Introduction

Materials and methods

Results

Conclusion

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Abstract

Introduction

Materials and methods

Results

Conclusion

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Leitlinien kompakt für die Innere Medizin

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Costims – das nächste heiße Ding in der Krebstherapie?

Perioperative Checkpointhemmer-Therapie verbessert NSCLC-Prognose

Positiver FIT: Die Ursache liegt nicht immer im Dickdarm

GLP-1-Agonisten können Fortschreiten diabetischer Retinopathie begünstigen

Update Innere Medizin